If despite several applications of simple methods no pregnancy occurs, the egg cell can be artificially fertilized in a test tube (in vitro fertilisation IVF). An IVF treatment is very complex and lasts several weeks.
To gain as many egg cells as possible at the same time, their production in the ovaries is stimulated with hormones. The doctor controls the size and maturity of the follicular cyst in the ovaries with ultra sound and hormone tests several times after hormone treatment. As soon as the egg cells seem ready to be fertilized, ovulation is induced through an injection of a further hormone. The fertilizable egg cells are taken out of the vagina 36 hours later with the help of long fine needle.
On the same day, fresh semen from the partner is prepared in the laboratory, placed together with the egg cells and cultivated in an incubator. If fertilization of one or several egg cells is successful, the doctor injects the two-day-old embryo into the uterine cavity of the woman with the help of a thin catheter.
About two weeks later, the doctor tests whether the female body has accepted the pregnancy, using a blood sample. There is a continuous risk of loss of pregnancy in the following weeks.